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Literature summary extracted from

  • Fraser, I.H.; Wadden, P.; Mookerjea, S.
    Purification and stabilization of galactosyltransferase from serum and lysolecithin extracted microsomes (1980), Can. J. Biochem., 58, 878-884.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Rattus norvegicus
2.4.1.90 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.1.38 ammonium sulfate stabilizes during storage Rattus norvegicus
2.4.1.38 glycerol stabilizes during storage Rattus norvegicus
2.4.1.90 ammonium sulfate stabilizes during storage Rattus norvegicus
2.4.1.90 glycerol stabilizes during storage Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 alpha-lactalbumin
-
 Rattus norvegicus
2.4.1.38 EDTA
-
 Rattus norvegicus
2.4.1.90 alpha-lactalbumin
-
 Rattus norvegicus
2.4.1.90 EDTA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.0118
-
 UDPgalactose enzyme from liver microsomal membrane Rattus norvegicus
2.4.1.38 0.01205
-
 UDPgalactose enzyme from serum Rattus norvegicus
2.4.1.90 0.0118
-
 UDPgalactose enzyme from liver microsomal membrane Rattus norvegicus
2.4.1.90 0.01205
-
 UDPgalactose enzyme from serum Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.38 microsome
-
 Rattus norvegicus
-
-
2.4.1.38 soluble
-
 Rattus norvegicus
-
-
2.4.1.90 microsome
-
 Rattus norvegicus
-
-
2.4.1.90 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Mn2+ required Rattus norvegicus
2.4.1.90 Mn2+ required Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Rattus norvegicus
-
-
-
2.4.1.90 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38
-
 Rattus norvegicus
2.4.1.90
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 liver
-
 Rattus norvegicus
-
2.4.1.38 serum
-
 Rattus norvegicus
-
2.4.1.90 liver
-
 Rattus norvegicus
-
2.4.1.90 serum
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.38 -20°C, bovine serum albumin, stable for up to 60 d Rattus norvegicus
2.4.1.90 -20°C, bovine serum albumin, stable for up to 60 d Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDPgalactose + glucose in presence of alpha-lactalbumin Rattus norvegicus lactose + UDP
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Rattus norvegicus UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + glucose in presence of alpha-lactalbumin Rattus norvegicus lactose + UDP
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Rattus norvegicus UDP + N-acetyllactosamine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 6.5
-
-
 Rattus norvegicus
2.4.1.90 6.5
-
-
 Rattus norvegicus